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R&D Systems recombinant mouse activin receptor ib fc
Recombinant Mouse Activin Receptor Ib Fc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody+recombinant+mouse+cripto/Mouse+Cripto+Antibody/pmc10048735-313-21-30
Average 93 stars, based on 6 article reviews

recombinant mouse activin receptor ib fc - by Bioz Stars, 2026-09

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Article Title: Cripto regulates hematopoietic stem cells as a hypoxic-niche-related factor through cell surface receptor GRP78.
Article Snippet: Real-time PCR reactions were performed on 7900 HT Fast Real-Time PCR System (Applied Biosystems), and the findings were normalized using HPRT amplification. .. 342 Cell Stem Cell 9, 330–344, October 7, 2011 a2011 Elsevier Inc. Recombinant Protein and Blocking Antibody Recombinant mouse Cripto was obtained from R&D Systems . .. Anti-GRP78 blocking antibody (N-20) was obtained from Santa Cruz Biotechnology .

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Figure 1. Cripto Is Highly Expressed in LT-HSCs and Maintains the Reconstitution Ability of HSCs after Ex Vivo Culture (A) Quantitative Real-Time PCR analysis for Cripto expression in LT-HSC (CD34Flt3KSL), ST-HSC (CD34+Flt3KSL), LMPP (CD34+Flt3+KSL), Lineage, and Lineage+ cells. Each value is normalized to HPRT expression and mean ± SD are shown. Signiﬁcance is calculated against LT-HSC (n = 3). (B) Cell count for cultured cells with or without rmCripto. Thirty CD34KSL cells were directly sorted into SFEM media supplemented with 100 ng/ml of mSCF and hTPO with or without 500 ng/ml of rmCripto in 96-well plates. Absolute cell number was counted at day 7 (left) and day 14 (right) using Trypan blue (mean ± SD, n = 4). (C) Colony forming unit assay (CFU assay) for cultured cells. All colony assays were initiated by plating 30 CD34KSL cells and scoring colonies at day 7 (left) and day 14 (right) (mean ± SD, n = 8). The number of GEMM mixed colonies was signiﬁcantly increased after treatment with rmCripto (day 7: p = 0.0473, day 14: p = 0.0422). GEMM: granulocytes, erythrocytes, macrophages, with or without megakaryocytes; E: erythrocytes; GM: granulocytes and macrophages; G: granulocytes; M: macrophages. (D) Competitive repopulation assay using fresh or cultured cells. Results from 4 weeks (left) and 16 weeks (right) after transplantation are shown (n = 10). Each circle indicates one mouse and red bars indicate average chimerism. (E) Secondary transplantation. Each circle indicates one mouse and red bars indicate average chimerism. Results from 12 weeks after transplantation are shown (n = 8).

Journal: Cell stem cell

Article Title: Cripto regulates hematopoietic stem cells as a hypoxic-niche-related factor through cell surface receptor GRP78.

doi: 10.1016/j.stem.2011.07.016

Figure Lengend Snippet: Figure 1. Cripto Is Highly Expressed in LT-HSCs and Maintains the Reconstitution Ability of HSCs after Ex Vivo Culture (A) Quantitative Real-Time PCR analysis for Cripto expression in LT-HSC (CD34Flt3KSL), ST-HSC (CD34+Flt3KSL), LMPP (CD34+Flt3+KSL), Lineage, and Lineage+ cells. Each value is normalized to HPRT expression and mean ± SD are shown. Signiﬁcance is calculated against LT-HSC (n = 3). (B) Cell count for cultured cells with or without rmCripto. Thirty CD34KSL cells were directly sorted into SFEM media supplemented with 100 ng/ml of mSCF and hTPO with or without 500 ng/ml of rmCripto in 96-well plates. Absolute cell number was counted at day 7 (left) and day 14 (right) using Trypan blue (mean ± SD, n = 4). (C) Colony forming unit assay (CFU assay) for cultured cells. All colony assays were initiated by plating 30 CD34KSL cells and scoring colonies at day 7 (left) and day 14 (right) (mean ± SD, n = 8). The number of GEMM mixed colonies was signiﬁcantly increased after treatment with rmCripto (day 7: p = 0.0473, day 14: p = 0.0422). GEMM: granulocytes, erythrocytes, macrophages, with or without megakaryocytes; E: erythrocytes; GM: granulocytes and macrophages; G: granulocytes; M: macrophages. (D) Competitive repopulation assay using fresh or cultured cells. Results from 4 weeks (left) and 16 weeks (right) after transplantation are shown (n = 10). Each circle indicates one mouse and red bars indicate average chimerism. (E) Secondary transplantation. Each circle indicates one mouse and red bars indicate average chimerism. Results from 12 weeks after transplantation are shown (n = 8).

Article Snippet: 342 Cell Stem Cell 9, 330–344, October 7, 2011 a2011 Elsevier Inc. Recombinant Protein and Blocking Antibody Recombinant mouse Cripto was obtained from R&D Systems .

Techniques: Ex Vivo, Real-time Polymerase Chain Reaction, Expressing, Cell Counting, Cell Culture, Colony-forming Unit Assay, Transplantation Assay

Figure 2. GRP78 Is a Functional Receptor for Cripto and Can Distinguish between Different Types of HSC Subsets (A) GRP78 expression on CD34KSL cells. Flow cytometry analysis revealed two clearly separated populations in the CD34KSL compartment. A representative proﬁle is shown. (B) CFU assay for GRP78+ and GRP78HSCs cultured with or without rmCripto and/or N-20. All colony assays were initiated by plating 30 GRP78+ or GRP78HSCs and colonies were scored at day 7 (mean ± SD, n = 4). The number of GEMM mixed colonies was signiﬁcantly increased only when GRP78+HSCs were treated with rmCripto (p = 0.0013). (C) Representative pictures of cultured GRP78+HSCs from (B). Black bars indicate 1 mm. (D) Competitive reconstitution assay using GRP78+ and GRP78HSCs. Chimerism in PB is shown 1 to 4 months after transplantation from engrafted mice with fresh cells or cultured cells (mean ± SD, *p < 0.001, **p < 0.05, n = 8). Cr: rmCripto; N: N-20. (E) Lineage distribution in mice transplanted with freshly isolated GRP78+ or GRP78HSCs. Results from 4 weeks (above) and 16 weeks (below) are shown. Each bar represents analysis from one mouse.

Journal: Cell stem cell

Article Title: Cripto regulates hematopoietic stem cells as a hypoxic-niche-related factor through cell surface receptor GRP78.

doi: 10.1016/j.stem.2011.07.016

Figure Lengend Snippet: Figure 2. GRP78 Is a Functional Receptor for Cripto and Can Distinguish between Different Types of HSC Subsets (A) GRP78 expression on CD34KSL cells. Flow cytometry analysis revealed two clearly separated populations in the CD34KSL compartment. A representative proﬁle is shown. (B) CFU assay for GRP78+ and GRP78HSCs cultured with or without rmCripto and/or N-20. All colony assays were initiated by plating 30 GRP78+ or GRP78HSCs and colonies were scored at day 7 (mean ± SD, n = 4). The number of GEMM mixed colonies was signiﬁcantly increased only when GRP78+HSCs were treated with rmCripto (p = 0.0013). (C) Representative pictures of cultured GRP78+HSCs from (B). Black bars indicate 1 mm. (D) Competitive reconstitution assay using GRP78+ and GRP78HSCs. Chimerism in PB is shown 1 to 4 months after transplantation from engrafted mice with fresh cells or cultured cells (mean ± SD, *p < 0.001, **p < 0.05, n = 8). Cr: rmCripto; N: N-20. (E) Lineage distribution in mice transplanted with freshly isolated GRP78+ or GRP78HSCs. Results from 4 weeks (above) and 16 weeks (below) are shown. Each bar represents analysis from one mouse.

Article Snippet: 342 Cell Stem Cell 9, 330–344, October 7, 2011 a2011 Elsevier Inc. Recombinant Protein and Blocking Antibody Recombinant mouse Cripto was obtained from R&D Systems .

Techniques: Functional Assay, Expressing, Flow Cytometry, Colony-forming Unit Assay, Cell Culture, Reconstitution Assay, Transplantation Assay, Isolation

Figure 3. Cripto Signaling Is Involved in Akt Pathway and Upregulates Glycolysis-Related Proteins (A) Intracellular staining of phosphorylated Akt (Ser473) in GRP78+HSCs and GRP78HSCs treated with or without rmCripto. Representative ﬂow cytometry patterns are shown. Black lines in Fresh panel mean starting materials. Blue lines show control condition (mSCF and hTPO) and red lines show rmCripto-treated samples. Each faint-colored line represents isotype controls. Analyses were performed after 15 min, 90 min, 12 hr, and 36 hr in culture. (B) Intracellular staining of phosphorylated 4E-BP1 (Thr37/46). (C) Intracellular staining of phosphorylated S6 (Ser235/236). (D) Intracellular staining of phosphorylated Src (Tyr416). (E) Intracellular staining of Smad2/3 (Ser423/425). (F) Phosphorylated protein levels in Cripto-treated or nontreated GRP78+/GRP78HSCs. All data represent mean ﬂuorescence intensity (MFI) ± SD (n = 4, *p < 0.05, **p < 0.01).

Journal: Cell stem cell

Article Title: Cripto regulates hematopoietic stem cells as a hypoxic-niche-related factor through cell surface receptor GRP78.

doi: 10.1016/j.stem.2011.07.016

Figure Lengend Snippet: Figure 3. Cripto Signaling Is Involved in Akt Pathway and Upregulates Glycolysis-Related Proteins (A) Intracellular staining of phosphorylated Akt (Ser473) in GRP78+HSCs and GRP78HSCs treated with or without rmCripto. Representative ﬂow cytometry patterns are shown. Black lines in Fresh panel mean starting materials. Blue lines show control condition (mSCF and hTPO) and red lines show rmCripto-treated samples. Each faint-colored line represents isotype controls. Analyses were performed after 15 min, 90 min, 12 hr, and 36 hr in culture. (B) Intracellular staining of phosphorylated 4E-BP1 (Thr37/46). (C) Intracellular staining of phosphorylated S6 (Ser235/236). (D) Intracellular staining of phosphorylated Src (Tyr416). (E) Intracellular staining of Smad2/3 (Ser423/425). (F) Phosphorylated protein levels in Cripto-treated or nontreated GRP78+/GRP78HSCs. All data represent mean ﬂuorescence intensity (MFI) ± SD (n = 4, *p < 0.05, **p < 0.01).

Article Snippet: 342 Cell Stem Cell 9, 330–344, October 7, 2011 a2011 Elsevier Inc. Recombinant Protein and Blocking Antibody Recombinant mouse Cripto was obtained from R&D Systems .

Techniques: Staining, Cytometry, Control

$Figure 5. Endosteal Niche Component Cells Express Cripto and Play a Critical Role for Maintenance of Dormant HSCs (A) Flow cytometry analysis of cell surface Cripto on bone-associated niche cells. Cells isolated from bone fragments were subdivided into three different populations based on the expression of ALCAM and Sca-I within the CD31CD45Ter119 fraction, and the cell-membrane-associated form of Cripto protein on each subpopulation was detected using anti-Cripto antibody. (B) Experimental plan for experiments using N-20 antibody injection in vivo. N-20 or control goat IgG (500 mg/kg) was injected into 8-week-old C57BL/6 female mice ﬁve times in 2 weeks. (C) Flow cytometry analysis of GRP78+ and GRP78HSCs in cHSCs from injected mice. The means ± SD percentage of GRP78+CD34 or GRP78CD34 cells in the KSL population are shown (n = 4). (D) Flow cytometry analysis for GRP78+ and GRP78HSCs in eHSCs of injected mice. The means ± SD percentage of GRP78+CD34 or GRP78CD34 cells in the KSL population are shown (n = 4). See also Figure S4.$

Journal: Cell stem cell

Article Title: Cripto regulates hematopoietic stem cells as a hypoxic-niche-related factor through cell surface receptor GRP78.

doi: 10.1016/j.stem.2011.07.016

Figure Lengend Snippet: Figure 5. Endosteal Niche Component Cells Express Cripto and Play a Critical Role for Maintenance of Dormant HSCs (A) Flow cytometry analysis of cell surface Cripto on bone-associated niche cells. Cells isolated from bone fragments were subdivided into three different populations based on the expression of ALCAM and Sca-I within the CD31CD45Ter119 fraction, and the cell-membrane-associated form of Cripto protein on each subpopulation was detected using anti-Cripto antibody. (B) Experimental plan for experiments using N-20 antibody injection in vivo. N-20 or control goat IgG (500 mg/kg) was injected into 8-week-old C57BL/6 female mice ﬁve times in 2 weeks. (C) Flow cytometry analysis of GRP78+ and GRP78HSCs in cHSCs from injected mice. The means ± SD percentage of GRP78+CD34 or GRP78CD34 cells in the KSL population are shown (n = 4). (D) Flow cytometry analysis for GRP78+ and GRP78HSCs in eHSCs of injected mice. The means ± SD percentage of GRP78+CD34 or GRP78CD34 cells in the KSL population are shown (n = 4). See also Figure S4.

Article Snippet: 342 Cell Stem Cell 9, 330–344, October 7, 2011 a2011 Elsevier Inc. Recombinant Protein and Blocking Antibody Recombinant mouse Cripto was obtained from R&D Systems .

Techniques: Flow Cytometry, Isolation, Expressing, Membrane, Injection, In Vivo, Control

Figure 6. HIF-1a Knockout Mice Contain Reduced Frequency of GRP78+HSCs and Impaired Expression of Cripto (A) Comparable frequency of the central GRP78+HSCs in HIF-1a cKO mice and controls. Representative FACS pattern of HIF-1a+/+ (left) and HIF-1aD/D (right) mice are shown. All data represent gating on the KSL population. (B) Reduced frequency of the endosteal GRP78+HSCs in HIF-1a cKO mice. Representative FACS pattern of HIF-1a+/+ (left) and HIF-1aD/D (right) mice are shown. All data represent gating on the KSL population. (C) Reduced frequency of GRP78+HSCs in HIF-1a cKO mice. The graph shows mean ± SD (n = 3). (D) Decreased expression of Cripto mRNA in KSL population of HIF-1a cKO mice. The graph shows mean ± SD (n = 3). (E)DecreasedfrequencyoftheendostealnichepopulationsofHIF-1acKOmice.RepresentativeFACSpatternsofHIF-1a+/+(left)andHIF-1aD/D(right)miceareshown.

Journal: Cell stem cell

Article Title: Cripto regulates hematopoietic stem cells as a hypoxic-niche-related factor through cell surface receptor GRP78.

doi: 10.1016/j.stem.2011.07.016

Figure Lengend Snippet: Figure 6. HIF-1a Knockout Mice Contain Reduced Frequency of GRP78+HSCs and Impaired Expression of Cripto (A) Comparable frequency of the central GRP78+HSCs in HIF-1a cKO mice and controls. Representative FACS pattern of HIF-1a+/+ (left) and HIF-1aD/D (right) mice are shown. All data represent gating on the KSL population. (B) Reduced frequency of the endosteal GRP78+HSCs in HIF-1a cKO mice. Representative FACS pattern of HIF-1a+/+ (left) and HIF-1aD/D (right) mice are shown. All data represent gating on the KSL population. (C) Reduced frequency of GRP78+HSCs in HIF-1a cKO mice. The graph shows mean ± SD (n = 3). (D) Decreased expression of Cripto mRNA in KSL population of HIF-1a cKO mice. The graph shows mean ± SD (n = 3). (E)DecreasedfrequencyoftheendostealnichepopulationsofHIF-1acKOmice.RepresentativeFACSpatternsofHIF-1a+/+(left)andHIF-1aD/D(right)miceareshown.

Article Snippet: 342 Cell Stem Cell 9, 330–344, October 7, 2011 a2011 Elsevier Inc. Recombinant Protein and Blocking Antibody Recombinant mouse Cripto was obtained from R&D Systems .

Techniques: Knock-Out, Expressing

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